[ASAP] Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein

		BioNMR > Educational resources > Journal club
[ASAP] Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-16-2021, 06:18 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein

[ASAP] Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein

Biochemistry
DOI: 10.1021/acs.biochem.0c00961

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Revolutionizing Our Understanding of AmyloidogenicProteins by Cryo-Electron Microscopy Revolutionizing Our Understanding of AmyloidogenicProteins by Cryo-Electron Microscopy http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01084/20180111/images/medium/bi-2017-010843_0002.gif Biochemistry DOI: 10.1021/acs.biochem.7b01084 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/dLp3nVo6iXs More...	nmrlearner	Journal club	0	01-12-2018 05:28 AM
Research into Cryo-Electron Microscopy Lands Nobel Prize in Chemistry - Futurism http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcRBPhRCfzv-Znaq8tFDa2RywqioAEn4T20nidAR4QgJRB-4kVaotLlGtuexwds6AM7fjAwxQc7S Futurism <img alt="" height="1" width="1"> Research into Cryo-Electron Microscopy Lands Nobel Prize in Chemistry Futurism â??But the difference is there are quite a lot of structures in biology that were resistant, were recalcitrant to the other methods, like x-ray crystallography or nuclear magnetic resonance spectroscopy. So it has opened up essentially a kind of new ... A cold, clear view of life wins chemistry NobelScience Magazine Swiss wins 2017...	nmrlearner	Online News	0	10-05-2017 01:01 AM
Cryo-electron microscopy: Alzheimer's fibrils Cryo-electron microscopy: Alzheimer's fibrils http://www.spectroscopynow.com/common/images/thumbnails/15ec800ad6f.jpgA technique that can augment X-ray crystallographic and spectroscopic studies has been demonstrated to provide an atomic resolution in imaging of the amyloid fibrils present in Alzheimer's disease, thanks to European research. Read the rest at Spectroscopynow.com	nmrlearner	General	0	10-01-2017 06:06 AM
Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... - Yahoo Finance UK Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... - Yahoo Finance UK <img alt="" height="1" width="1"> Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... Yahoo Finance UK The report segments the global protein crystallography market based on technique employed, including X-ray crystallography, Electron crystallography, NMR spectroscopy, and others. The report also segments the global protein crystallization base on ... and more » Read here	nmrlearner	Online News	0	03-29-2016 01:43 AM
Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... - Business Wire (press release) Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... - Business Wire (press release) <img alt="" height="1" width="1"> Advances in Cryo-Electron Microscopy Will Improve the Global Protein Crystallization and Crystallography Market ... Business Wire (press release) The report segments the global protein crystallography market based on technique employed, including X-ray crystallography, Electron crystallography, NMR spectroscopy, and others. The report also segments the global protein crystallization base on ... and more...	nmrlearner	Online News	0	03-08-2016 03:00 AM
[NMR images] Other methods are Cryo-EM(electron microscopy), http://3.bp.blogspot.com/_H3VvAQred7I/SSRk1W97JBI/AAAAAAAAAII/G32vZntGdTE/s400/NMR.jpg 7/06/2014 7:13:57 PM GMT Other methods are Cryo-EM(electron microscopy), More...	nmrlearner	NMR pictures	0	06-07-2014 07:12 PM
[NMR tweet] cryo-electron microscopy, nuclear magnetic resonance spectroscopy, cryo-EM tomography. cryo-electron microscopy, nuclear magnetic resonance spectroscopy, cryo-EM tomography. Published by xFREEMINDx (Prestøn) on 2013-05-31T19:42:44Z Source: Twitter	nmrlearner	Twitter NMR	0	05-31-2013 08:11 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off