[ASAP] Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR

		BioNMR > Educational resources > Journal club
[ASAP] Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-25-2022, 07:54 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR

[ASAP] Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR

Carl O?ster, Kumar Tekwani Movellan, Benjamin Goold, Kitty Hendriks, Sascha Lange, Stefan Becker, Bert L. de Groot, Wojciech Kopec, Loren B. Andreas, and Adam Lange

Journal of the American Chemical Society
DOI: 10.1021/jacs.1c13247

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions #DNPNMR From The DNP-NMR Blog: Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions #DNPNMR Jardón-Álvarez, Daniel, Guy Reuveni, Adi Harchol, and Michal Leskes. “Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions.” The Journal of Physical Chemistry Letters 11, no. 14 (July 16, 2020): 5439–45. https://doi.org/10.1021/acs.jpclett.0c01527.	nmrlearner	News from NMR blogs	0	10-19-2020 05:07 PM
[NMR paper] Solid phase synthesis, NMR structure determination of ?-KTx3.8, its in silico docking to Kv1.x potassium channels, and electrophysiological analysis provide insights into toxin-channel selectivity. Solid phase synthesis, NMR structure determination of ?-KTx3.8, its in silico docking to Kv1.x potassium channels, and electrophysiological analysis provide insights into toxin-channel selectivity. Related Articles Solid phase synthesis, NMR structure determination of ?-KTx3.8, its in silico docking to Kv1.x potassium channels, and electrophysiological analysis provide insights into toxin-channel selectivity. Toxicon. 2015 May 5; Authors: Kohl B, Rothenberg I, Ali SA, Alam M, Seebohm G, Kalbacher H, Voelter W, Stoll R Abstract ...	nmrlearner	Journal club	0	05-10-2015 03:50 AM
[NMR paper] An NMR investigation of the structure, function and role of the hERG channel selectivity filter in the long QT syndrome. An NMR investigation of the structure, function and role of the hERG channel selectivity filter in the long QT syndrome. Related Articles An NMR investigation of the structure, function and role of the hERG channel selectivity filter in the long QT syndrome. Biochim Biophys Acta. 2013 Mar 5; Authors: Gravel AE, Arnold AA, Dufourc EJ, Marcotte I Abstract The human ether-a-go-go-related gene (hERG) voltage-gated K+ channels are located in heart cell membranes and hold a unique selectivity filter (SF) amino acid sequence (SVGFG) as compared...	nmrlearner	Journal club	0	03-12-2013 07:09 PM
Direct detection of nitrogen-14 in solid-state NMR spectroscopy Direct detection of nitrogen-14 in solid-state NMR spectroscopy November 2011 Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 4</br> </br> Highlights	nmrlearner	Journal club	0	12-15-2012 09:51 AM
Direct detection of nitrogen-14 in solid-state NMR spectroscopy Direct detection of nitrogen-14 in solid-state NMR spectroscopy November 2011 Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 4</br> </br> Highlights	nmrlearner	Journal club	0	12-01-2012 06:10 PM
Direct detection of nitrogen-14 in solid-state NMR spectroscopy Direct detection of nitrogen-14 in solid-state NMR spectroscopy Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 4</br> Luke A. O’Dell</br> </br> </br></br>	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 20 April 2011</br> Luke A., O’Dell</br> Highlights:? Experimental methods for the direct detection of 14N are surveyed ? Advantages, disadvantages and practicalities of each technique are discussed ? Includes single-crystal, ultra-wideline, MAS and overtone spectroscopy</br></br> More...	nmrlearner	Journal club	0	04-21-2011 03:00 PM
[NMR paper] 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of he 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of heme proteins. Related Articles 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of heme proteins. J Am Chem Soc. 2002 May 29;124(21):5936-7 Authors: Fujii H 13CN ion appears to have the greatest potential to probe the heme environment of the ferric heme proteins; however, a resonance of the iron-bound (13)CN ion in ferric heme proteins has not yet been located. We show here the first detection of (13)C NMR signals of the...	nmrlearner	Journal club	0	11-24-2010 08:49 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off