[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells

		BioNMR > Educational resources > Journal club
[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-30-2020, 02:58 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells

[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells

Biochemistry
DOI: 10.1021/acs.biochem.0c00281

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] Target Engagement and Binding Mode of an Antituberculosis Drug to Its Bacterial Target Deciphered in Whole Living Cells by NMR Target Engagement and Binding Mode of an Antituberculosis Drug to Its Bacterial Target Deciphered in Whole Living Cells by NMR https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00975/20190103/images/medium/bi-2018-00975t_0006.gif Biochemistry DOI: 10.1021/acs.biochem.8b00975 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/J0ajkYKpz7s More...	nmrlearner	Journal club	0	01-07-2019 05:49 AM
[ASAP] Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00917/20181114/images/medium/bi-2018-009172_0004.gif Biochemistry DOI: 10.1021/acs.biochem.8b00917 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/teMgPiYQoO4 More...	nmrlearner	Journal club	0	11-25-2018 06:02 AM
[NMR paper] Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells. Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells. Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells. J Med Chem. 2018 Mar 09;: Authors: Brancaccio D, Diana D, Di Maro S, Di Leva FS, Tomassi S, Fattorusso R, Russo L, Scala S, Trotta AM, Portella L, Novellino E, Marinelli L, Carotenuto A Abstract Peptides-binding G protein-coupled receptors play an important role in many pathological and physiological pathways....	nmrlearner	Journal club	0	03-10-2018 04:36 PM
[NMR paper] Fluorine NMR-based assay in living mammalian cells. Fluorine NMR-based assay in living mammalian cells. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Fluorine NMR-based assay in living mammalian cells. Anal Biochem. 2015 Dec 11; Authors: Veronesi M, Giacomina F, Romeo E, Castellani B, Ottonello G, Lambruschini C, Garau G, Scarpelli R, Bandiera T, Piomelli D, Dalvit C Abstract Nuclear Magnetic Resonance (NMR)-based screening has been recognized as a powerful approach for the identification...	nmrlearner	Journal club	0	12-28-2015 12:26 AM
Detection of RNA–Protein Interactions in Living Cells with SHAPE Detection of RNA–Protein Interactions in Living Cells with SHAPE http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b00977/20151111/images/medium/bi-2015-00977x_0007.gif Biochemistry DOI: 10.1021/acs.biochem.5b00977 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/WwxMghExNKY More...	nmrlearner	Journal club	0	11-12-2015 09:05 AM
[NMR images] NMR protein structure determination in living E. coli cells using ... http://www.nature.com/nprot/journal/v5/n6/images/nprot.2010.69-I2.gif 7/06/2014 7:13:57 PM GMT NMR protein structure determination in living E. coli cells using ... More...	nmrlearner	NMR pictures	0	06-07-2014 07:12 PM
[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy. Protein dynamics in living cells studied by in-cell NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy. FEBS Lett. 2013 Jan 11; Authors: Li C, Liu M Abstract Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...	nmrlearner	Journal club	0	02-03-2013 10:22 AM
13C direct-detection biomolecular NMR spectroscopy in living cells. 13C direct-detection biomolecular NMR spectroscopy in living cells. 13C direct-detection biomolecular NMR spectroscopy in living cells. Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2339-41 Authors: Bertini I, Felli IC, Gonnelli L, Kumar M V V, Pierattelli R	nmrlearner	Journal club	0	06-18-2011 01:10 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off