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Side-chains:
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UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
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Refinement:
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Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
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MICS caps, β-turns
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Flexibility from chemical shifts:
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Interactions from chemical shifts:
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Chemical shifts re-referencing:
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LACS
CheckShift
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Chemical shifts:
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RDCs:
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Anisofit
Pseudocontact shifts:
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Protein geomtery:
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PROSESS
What-If
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PSVS
MolProbity
SAVES2 or SAVES4
Vadar
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MetaMQAPII
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Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
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V-NMR
Flexibility from structure:
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Methyl S2
B-factor
Molecular dynamics:
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Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
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CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


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Old 05-09-2007, 10:15 AM
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Default Protein chemist position is available

Posting the following job ad was requested by Dr. Wishart lab.

PROTEIN CHEMIST
Dr. Wishart's research group
University of Alberta



Position Type: Full Time - Grant Funded

Salary range: $3544 - $4492 CAD per month
Grade: 9
Hours: 35 per wk
This position has a comprehensive benefits program.

Duties

* Designs and prepares various hamster prion gene constructs (site-directed mutants, deletions) in E. coli; responsible for their expression, purification, stable isotope labeling (for NMR and MS analysis) and refolding
* Implements protein-misfolding cyclic amplification (PMCA) assay
* Undertakes primer, plasmid and peptide design and construction as directed
* Oversees bacterial, oligonucleotide and plasmid stocks; maintains complete records
* Prepares and maintains chemicals, media and stock solutions; operates laboratory equipment in safe and efficient manner
* Reports, unloads or annotates results into laboratory management system
* Interacts with and responds to general protein production needs of affiliated project researchers

Qualifications

* B.Sc. or M.Sc. degree in biology, biochemistry (specialization in molecular biology, microbiology or biochemistry)
* Minimum 2 years experience in a research laboratory; previous experience with chemical protein modification or with electron microscopy an asset
* Computer proficiency using MS Office applications (Word, Excel, Outlook)
* Willingness to travel and train in various laboratories across North America

Contact:

Treena Neufeld
Faculty Assistant to Dr. David Wishart
Phone: (780) 492-4846
Fax: (780) 492-1071
E-mail: tneufeld@ualberta.ca


Lab website: web. address: http://wishart.biology.ualberta.ca/
Prion project website: http://www.prionproject.ca/
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