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Site-Directed Methyl Group Labeling as an NMR Probe of Structure and Dynamics in Supramolecular Protein Systems: Applications to the Proteasome and to the ClpP Protease
Site-Directed Methyl Group Labeling as an NMR Probe of Structure and Dynamics in Supramolecular Protein Systems: Applications to the Proteasome and to the ClpP Protease
Tomasz L. Religa, Amy M. Ruschak, Rina Rosenzweig and Lewis E. Kay
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja202259a/aop/images/medium/ja-2011-02259a_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja202259a
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/rQfCMlQFoW8
[NMR paper] Structure-based analysis of protein dynamics: comparison of theoretical results for h
Structure-based analysis of protein dynamics: comparison of theoretical results for hen lysozyme with X-ray diffraction and NMR relaxation data.
Related Articles Structure-based analysis of protein dynamics: comparison of theoretical results for hen lysozyme with X-ray diffraction and NMR relaxation data.
Proteins. 1999 Dec 1;37(4):654-67
Authors: Haliloglu T, Bahar I
An analytical approach based on Gaussian network model (GNM) is proposed for predicting the rotational dynamics of proteins. The method, previously shown to successfully...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
[NMR thesis] Nuclear spin dynamics and thermodynamics of pulsed NMR in solids
Nuclear spin dynamics and thermodynamics of pulsed NMR in solids
Burum, Douglas Peter (1979) Nuclear spin dynamics and thermodynamics of pulsed NMR in solids. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-04302007-153624
More...
nmrlearner
NMR theses
0
08-27-2010 01:45 AM
Spin Choreography vs Spin Dynamics
http://www.bionmr.com/uploads/Freeman.jpg
Has anybody read Spin Choreography: Basic Steps in High Resolution NMR by Ray Freemanhttp://www.assoc-amazon.com/e/ir?t=bionmr-20&l=ur2&o=1? Is it better, worse or just different than Spin Dynamics by Malcom Levitthttp://www.assoc-amazon.com/e/ir?t=bionmr-20&l=ur2&o=1?
nmrlearner
Books
1
04-20-2006 11:46 AM
2H NMR Spin Relaxation for studying RNA dynamics
A Suite of 2H NMR Spin Relaxation Experiments for the Measurement of RNA Dynamics
Pramodh Vallurupalli and Lewis E. Kay, J. Am. Chem. Soc.; 2005; 127 (18) pp 6893 - 6901
ABSTRACT:
A suite of (2)H-based spin relaxation NMR experiments is presented for the measurement of molecular dynamics in a site-specific manner in uniformly (13)C, randomly fractionally deuterated ( approximately 50%) RNA molecules. The experiments quantify (2)H R(1) and R(2) relaxation rates that can subsequently be analyzed to obtain information about dynamics on a pico- to nanosecond time scale. Sensitivity...
nmrlearner
Journal club
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05-19-2005 08:44 AM
DYNAMICS, RELAXFIT & ROTDIF from Dr. Fushman group
DYNAMICS, RELAXFIT & ROTDIF, programs for analysis of 15N NMR relaxation experiments, are currently available for download from the website of Dr. Fushman's group . Below are the software description and references that were borrowed from Dr. Fushman's website. RELAXFIT
A program for extracting relaxation rates (e.g. R1 or R2) from fitting experimental data to an exponentially decaying function.
Reference: Fushman et al., J. Mol. Biol. 266 (1997) 173.
DYNAMICS
A program for model-free analysis of 15N relaxation data.
Features isotropic, axially-symmetric and fully anisotropic models...
nmrlearner
NMR software
0
04-08-2005 06:47 AM
A new spin probe of protein dynamics
A new spin probe of protein dynamics: nitrogen relaxation in (15)n-(2)h amide groups.
Xu J, Millet O, Kay LE, Skrynnikov NR.
Contribution from the Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, and Departments of Medical Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
J Am Chem Soc. 2005 Mar 9;127(9):3220-9.
(15)N spin relaxation data have provided a wealth of information on protein dynamics in solution. Standard R(1), R(1)(rho), and NOE experiments aimed at (15)N amide moieties are complemented in this...