[Question from NMRWiki Q&A forum] Concentration-dependent signal fine splitting
Concentration-dependent signal fine splitting
Hi,
I have synthesized coproporphyrin II tetramethyl ester and taken a 1H NMR in CDCl3:
http://illumina-chemie.de/upload/5_11600286385136157fa7971.png
Especially the peaks at 10.1 ppm, belonging to the CH groups connecting the pyrrole rings, show a strong concentration dependence. They should be two singlets, but in fact they are only singlets at very low concentrations and split up with increasing concentration:
nmrlearner
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Linear Mode
