Researchers at the University of Manchester, UK, and their colleagues, have developed an ultra-high-resolution NMR spectroscopy technique, which they have given the wonderful acronym PSYCHE, for pure shift yielded by chirp excitation.
Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot
From the The DNP-NMR Blog:
Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot
Safiullin, K., C. Talbot, and P.J. Nacher, Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot. J Magn Reson, 2013. 227(0): p. 72-86.
http://dx.doi.org/10.1016/j.jmr.2012.11.025
nmrlearner
News from NMR blogs
0
04-15-2013 08:52 AM
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
Abstract While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decreasing extent of ordered structure, this task often becomes both difficult and time-consuming, and the recording of high-dimensional data with high-resolution may be essential. Random sampling of the evolution time space,...
nmrlearner
Journal club
0
07-20-2012 11:13 PM
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
Abstract Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase...
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
Abstract Magic-angle spinning (MAS) solid-state NMR (SSNMR) spectroscopy of uniformly-13C,15N labeled protein samples provides insight into atomic-resolution chemistry and structure. Data collection efficiency has advanced remarkably in the last decade; however, the study of larger proteins is still challenged by relatively low resolution in comparison to solution NMR. In this study, we present a systematic analysis of SSNMR protein spectra acquired at 11.7, 17.6 and 21.1 Tesla (1H frequencies of...
nmrlearner
Journal club
0
01-09-2011 12:46 PM
[NMR paper] A high-resolution technique for multidimensional NMR spectroscopy.
A high-resolution technique for multidimensional NMR spectroscopy.
Related Articles A high-resolution technique for multidimensional NMR spectroscopy.
IEEE Trans Biomed Eng. 1998 Jan;45(1):78-86
Authors: Li Y, Razavilar J, Liu KJ
In this paper, a scheme for estimating frequencies and damping factors of multidimensional nuclear magnetic resonance (NMR) data is presented, multidimensional NMR data can be modeled as the sum of several multidimensional damped sinusoids. The estimated frequencies and damping factors of multidimensional NMR data...
nmrlearner
Journal club
0
11-17-2010 11:06 PM
[NMR paper] High-resolution NMR of biological solids.
High-resolution NMR of biological solids.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles High-resolution NMR of biological solids.
Curr Opin Struct Biol. 1996 Oct;6(5):624-9
Authors: McDowell LM, Schaefer J
Solid-state NMR experiments have recently provided a number of biochemical insights: motionally averaged 2H lineshapes have shown that the motion of a backbone loop protecting a protein binding site is not ligand gated; isotropic 13C chemical shifts of...
PSYCHE NMR: High-resolution not all in the mind
Researchers at the University of Manchester, UK, and their colleagues, have developed an ultra-high-resolution NMR spectroscopy technique, which they have given the wonderful acronym PSYCHE, for pure shift yielded by chirp excitation.
Linear Mode
