[NMR paper] NMR studies of protein folding and binding in cells and cell-like environments.
NMR studies of protein folding and binding in cells and cell-like environments.
NMR studies of protein folding and binding in cells and cell-like environments.
Curr Opin Struct Biol. 2014 Dec 2;30C:7-16
Authors: Smith AE, Zhang Z, Pielak GJ, Li C
Abstract
Proteins function in cells where the concentration of macromolecules can exceed 300g/L. The ways in which this crowded environment affects the physical properties of proteins remain poorly understood. We summarize recent NMR-based studies of protein folding and binding...
nmrlearner
Journal club
0
12-06-2014 05:18 PM
NMR studies of protein folding and binding in cells and cell-like environments
NMR studies of protein folding and binding in cells and cell-like environments
Publication date: February 2015
Source:Current Opinion in Structural Biology, Volume 30</br>
Author(s): Austin E Smith , Zeting Zhang , Gary J Pielak , Conggang Li</br>
Proteins function in cells where the concentration of macromolecules can exceed 300g/L. The ways in which this crowded environment affects the physical properties of proteins remain poorly understood. We summarize recent NMR-based studies of protein folding and binding conducted in cells and in vitro under crowded...
nmrlearner
Journal club
0
12-04-2014 04:37 AM
[NMR paper] 19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate.
19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate.
19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate.
Biochemistry. 2013 Aug 1;
Authors: Kitevski-Leblanc JL, Hoang J, Thach W, Larda ST, Prosser RS
Abstract
While many proteins are recognized to undergo folding via an intermediate, the microscopic nature of folding intermediates is less understood. In this study, 19F NMR and near UV circular dichroism (CD) are used to characterize a transition to a thermal folding intermediate of calmodulin, a...
nmrlearner
Journal club
0
08-04-2013 03:18 AM
[NMR paper] NMR spectroscopic characterization of millisecond protein folding by transverse relaxation dispersion measurements.
NMR spectroscopic characterization of millisecond protein folding by transverse relaxation dispersion measurements.
Related Articles NMR spectroscopic characterization of millisecond protein folding by transverse relaxation dispersion measurements.
J Am Chem Soc. 2005 Sep 28;127(38):13207-12
Authors: Zeeb M, Balbach J
The cold shock protein CspB adopts its native and functional tertiary structure on the millisecond time scale. We employed transverse relaxation NMR methods, which allow a quantitative measurement of the cooperativity of this...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
[NMR paper] Millisecond protein folding studied by NMR spectroscopy.
Millisecond protein folding studied by NMR spectroscopy.
Related Articles Millisecond protein folding studied by NMR spectroscopy.
Protein Pept Lett. 2005 Feb;12(2):139-46
Authors: Zeeb M, Balbach J
Proteins are involved in virtually every biological process and in order to function, it is necessary for these polypeptide chains to fold into the unique, native conformation. This folding process can take place rapidly. NMR line shape analyses and transverse relaxation measurements allow protein folding studies on a microsecond-to-millisecond...
nmrlearner
Journal club
0
11-24-2010 11:14 PM
[NMR paper] Kinetic studies of protein folding using NMR spectroscopy.
Kinetic studies of protein folding using NMR spectroscopy.
Related Articles Kinetic studies of protein folding using NMR spectroscopy.
Nat Struct Biol. 1998 Jul;5 Suppl:504-7
Authors: Dobson CM, Hore PJ
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[NMR paper] NMR and protein folding: equilibrium and stopped-flow studies.
NMR and protein folding: equilibrium and stopped-flow studies.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR and protein folding: equilibrium and stopped-flow studies.
Protein Sci. 1993 Dec;2(12):2007-14
Authors: Frieden C, Hoeltzli SD, Ropson IJ
NMR studies are now unraveling the structure of intermediates...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] Prelude to NMR studies of protein folding.
Prelude to NMR studies of protein folding.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_nsb.gif Related Articles Prelude to NMR studies of protein folding.
Nat Struct Biol. 1999 Jul;6(7):608
Authors: Smith T
Millisecond Protein Folding Studies by NMR Spectroscopy
Linear Mode
