A simple 31P NMR tube experiment has been developed that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase.
Quantifying reaction kinetics of the non-enzymatic decarboxylation of pyruvate and production of peroxymonocarbonate with hyperpolarized 13C-NMR
From The DNP-NMR Blog:
Quantifying reaction kinetics of the non-enzymatic decarboxylation of pyruvate and production of peroxymonocarbonate with hyperpolarized 13C-NMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Drachman, N., et al., Quantifying reaction kinetics of the non-enzymatic decarboxylation of pyruvate and production of peroxymonocarbonate with hyperpolarized 13C-NMR. Phys. Chem. Chem. Phys., 2017. 19(29): p. 19316-19325.
http://dx.doi.org/10.1039/C7CP02041D
nmrlearner
News from NMR blogs
0
11-23-2017 08:38 AM
Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization
From The DNP-NMR Blog:
Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization
Miclet E, Abergel D, Bornet A, Milani J, Jannin S, Bodenhausen G. Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization. The Journal of Physical Chemistry Letters. 2014;5(19):3290-5.
http://dx.doi.org/10.1021/jz501411d
nmrlearner
News from NMR blogs
0
06-03-2015 11:04 PM
Hyperpolarized [1,(13)C]pyruvate in lysed human erythrocytes: effects of co-substrate supply on reaction time courses
From The DNP-NMR Blog:
Hyperpolarized pyruvate in lysed human erythrocytes: effects of co-substrate supply on reaction time courses
Pages, G., Y.L. Tan, and P.W. Kuchel, Hyperpolarized pyruvate in lysed human erythrocytes: effects of co-substrate supply on reaction time courses. NMR Biomed, 2014. 27(10): p. 1203-10.
http://www.ncbi.nlm.nih.gov/pubmed/25111006
[Question from NMRWiki Q&A forum] demonstrative kinetics experiment
demonstrative kinetics experiment
I'm looking for a good kinetics experiment to run in the lab section of our graduate lab course. It should be a reaction that we can set up on a bench with inexpensive materials, that will take place safely in an NMR tube, and should take about one to five hours to complete at room temperature. (If done in one, then the students can witness the outcome; more than that, and they'll just set it up, watch the first few minutes of it, then leave and get the data later.) We can follow it using any high-sensitivity high-natural abundance nucleus: 1H, 19F, or...
nmrlearner
News from other NMR forums
0
11-21-2011 08:06 AM
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Nat Chem Biol. 2011 Mar 20;
Authors: Ullrich SJ, Hellmich UA, Ullrich S, Glaubitz C
The simultaneous observation of interdependent reactions within different phases as catalyzed by membrane-bound enzymes is still a challenging task. One such enzyme, the Escherichia coli integral membrane protein diacylglycerol kinase (DGK), is a key player in lipid regulation. It catalyzes the...
nmrlearner
Journal club
0
03-23-2011 05:41 PM
[NMR paper] 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid form
13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis.
Related Articles 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis.
Biotechnol Prog. 2000 Mar-Apr;16(2):169-75
Authors: Phalakornkule C, Fry B, Zhu T, Kopesel R, Ataai MM, Domach MM
When batch and continuous Bacillus subtilis cultures are provided with a small amount of citrate, acid production ceases, carbon yield increases by more than 2-fold, and the productivity of...
nmrlearner
Journal club
0
11-18-2010 09:15 PM
[NMR paper] Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optic
Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optical spectroscopy.
Related Articles Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optical spectroscopy.
J Mol Biol. 1998 Feb 27;276(3):657-67
Authors: Guijarro JI, Morton CJ, Plaxco KW, Campbell ID, Dobson CM
The refolding kinetics of the chemically denatured SH3 domain of phosphatidylinositol 3'-kinase (PI3-SH3) have been monitored by real-time one-dimensional 1H NMR coupled with a variety of other biophysical techniques. These...
Journal Highlight: 31P NMR of the pyruvate kinase reaction: An undergraduate experiment in enzyme kinetics
A simple 31P NMR tube experiment has been developed that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase.
Linear Mode
