Below is a Google-cached version of Protein NMR - A Practical Guide page from crashed protein-nmr.org.uk. You may want to check first if the site was restored.
Most books on Protein NMR focus on theoretical aspects and pulse sequences with only little space devoted to resonance assignment and structure calculations. At the same time many software manuals provide detailed information on how to use the software, but assume prior knowledge of the concepts of assignment and structure calculation. This has produced a gap in this area which these webpages aim to bridge by describing the concepts of assignment (and in future also structure calculations) in detail with the help of many illustrations. Much space and discussion is devoted to practical aspects.
The implementation of protein NMR assignment is described using the program
CCPNmr Analysis. This is a comparitively new program which is being developed by
CCPN and actively seeks input from the NMR community. CCPNmr Analysis is based on the detailed and well thought-out
CCPN Data Model which has the advantage (a) that it feeds directly into the CCPN Format Converter thus simplifying the import from and export to other programs, and (b) that as more and more NMR-related programs adopt the CCPN Data Model it is likely to take on a key role in NMR data management - in a similar way to
CCP4 for protein X-ray crystallography. CCPNmr Analysis is already one of the best assignment programs availabe while still being developed and provides excellent support via the
CCPN Mailing List (a
manual is also available).
Webpages include:
- description of several resonance assignment strategies
- simple descriptions and discussions of many multidimensional NMR experiments commonly used in protein NMR
- basic usage of CCPNmr Analysis (versions 1 and 2)
- advice for using CCPNmr Analysis with solid-state MAS NMR data
- tutorial on protein assignment using solid-state MAS NMR data
- description of isotopic labelling strategies commonly used in protein NMR
- links to a large number of protein NMR software packages
- suggested literature for further reading
- links to other useful NMR webpages
- Glossary
Future developments:
- introduction to chemical shifts - their properties and uses
- homonuclear assignment strategies for unlabelled proteins and peptides
- basic principles of protein structure calculation using NMR data
