NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.
Exceeding the limit of dynamics studies on biomolecules using high spin-lock field strengths with a cryogenically cooled probehead
Exceeding the limit of dynamics studies on biomolecules using high spin-lock field strengths with a cryogenically cooled probehead
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
David Ban, Alvar D. Gossert, Karin Giller, Stefan Becker, Christian Griesinger, Donghan Lee</br>
Internal motions in the microsecond timescale have been proposed to play an active part in a protein’s biological function. Nuclear magnetic resonance (NMR) relaxation dispersion is a robust method sensitive to this timescale with atomic resolution. However, due to technical...
nmrlearner
Journal club
0
05-15-2012 06:40 PM
Dynamics of Biomolecules from Picoseconds to Seconds at Atomic Resolution
Dynamics of Biomolecules from Picoseconds to Seconds at Atomic Resolution
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 22 July 2011</br>
Dennis A., Torchia</br>
Although biomolecular dynamics has been investigated using NMR for at least 40 years, only in the past 20 years have internal motions been characterized at atomic resolution throughout proteins and nucleic acids. This development was made possible by multidimensional heteronuclear NMR approaches that provide near complete sequential signal assignments of...
nmrlearner
Journal club
0
07-23-2011 10:40 PM
Methods to determine slow diffusion coefficients of biomolecules. Applications to Engrailed 2, a partially disordered protein
Methods to determine slow diffusion coefficients of biomolecules. Applications to Engrailed 2, a partially disordered protein
Abstract We present new NMR methods to measure slow translational diffusion coefficients of biomolecules. Like the heteronuclear stimulated echo experiment (XSTE), these new methods rely on the storage of information about spatial localization during the diffusion delay as longitudinal polarization of nuclei with long T1 such as nitrogen-15. The new BEST-XSTE sequence combines features of Band-selective Excitation Short-Transient (BEST) and XSTE methods. By...
nmrlearner
Journal club
0
05-24-2011 10:00 AM
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57
Authors: Renault M, Cukkemane A, Baldus M
Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into complex biomolecular systems ranging from lipid bilayers to complex biomaterials. In the...
nmrlearner
Journal club
0
02-15-2011 07:17 PM
[NMR paper] Partial alignment of biomolecules: an aid to NMR characterization.
Partial alignment of biomolecules: an aid to NMR characterization.
Related Articles Partial alignment of biomolecules: an aid to NMR characterization.
Curr Opin Chem Biol. 2001 Oct;5(5):584-90
Authors: Prestegard JH, Kishore AI
Partial alignment of biomolecules in solution has added a new dimension to structural investigation by high-resolution NMR methods. Applications to proteins, nucleic acids and carbohydrates now abound. Limitations initially associated with compatibility of biomolecules with the liquid-crystal media commonly used to...
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[NMR paper] Practical methods for solid-state NMR distance measurements on large biomolecules: co
Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance.
Related Articles Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance.
J Magn Reson. 1999 Aug;139(2):371-6
Authors: Balazs YS, Thompson LK
Simple modifications of the rotational resonance experiment substantially reduce the total experimental time needed to measure weak homonuclear dipolar couplings, a critical factor for achieving routine internuclear distance...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
[NMR paper] Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-sci_full_freeReg.gif Related Articles Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
Science. 1997 May 9;276(5314):930-2
Authors: Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG
Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle...
nmrlearner
Journal club
0
08-22-2010 03:31 PM
[NMR paper] Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-sci_full_freeReg.gif Related Articles Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
Science. 1997 May 9;276(5314):930-2
Authors: Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG
Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle...
NMR of Biomolecules
NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.
Linear Mode
