Thread: NMR paper Mapping invisible epitopes by NMR spectroscopy.
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Default Mapping invisible epitopes by NMR spectroscopy.
Mapping invisible epitopes by NMR spectroscopy.

Related Articles Mapping invisible epitopes by NMR spectroscopy.

J Biol Chem. 2020 Dec 18;295(51):17411-17412

Authors: Usher ET, Showalter SA

Abstract
Defining discontinuous antigenic epitopes remains a substantial challenge, as exemplified by the case of lipid transfer polyproteins, which are common pollen allergens. Hydrogen/deuterium exchange monitored by NMR can be used to map epitopes onto folded protein surfaces, but only if the complex rapidly dissociates. Modifying the standard NMR-exchange measurement to detect substoichiometric complexes overcomes this time scale limitation and provides new insights into recognition of lipid transfer polyprotein by antibodies. In the future, this new and exciting development should see broad application to a range of tight macromolecular interactions.


PMID: 33453987 [PubMed - in process]



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