RNA characterization by solid-state NMR spectroscopy.
Related Articles RNA characterization by solid-state NMR spectroscopy.
Chemistry. 2017 Dec 25;:
Authors: Yang Y, Wang S
Abstract
The structures of RNAs, which play critical roles in various biological processes, provide important clues and insights into the biological functions of these molecules. However, RNA structure determination remains a challenging topic. In recent years, magic-angle-spinning solid-state nuclear magnetic resonance (MAS SSNMR) has emerged as an alternative technique for structural and dynamic characterization of RNA. MAS SSNMR has been successfully applied to provide atomic-level structural information about several RNA molecules and RNA-protein complexes. In this Minireview, we give an overview of recent progress in the field of MAS SSNMR based RNA structural characterization and introduce
sample preparation strategies and SSNMR spectroscopic techniques that have been incorporated to identify structural elements. We also highlight a few impressive examples of RNAs that have been investigated extensively by SSNMR. Finally, we briefly discuss future technical trends in the use of MAS SSNMR to facilitate RNA structure determination.
PMID: 29277936 [PubMed - as supplied by publisher]
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