Determination of the binding epitope of RGD-peptidomimetics to ?v?3 and ?(IIb)?3 integrin-rich intact cells by NMR and computational studies.
Related Articles Determination of the binding epitope of RGD-peptidomimetics to ?v?3 and ?(IIb)?3 integrin-rich intact cells by NMR and computational studies.
Org Biomol Chem. 2013 Jun 21;11(23):3886-93
Authors: Guzzetti I, Civera M, Vasile F, Araldi EM, Belvisi L, Gennari C, Potenza D, Fanelli R, Piarulli U
Abstract
NMR experiments (transferred NOE and Saturation Transfer Difference) were used to shed light on the binding epitope of RGD peptidomimetics 1-3 with integrins ?v?3 and ?(IIb)?3, expressed on the membrane of ECV304 bladder cancer cells and human platelets, respectively. The NMR results were supported by docking calculations of 1-3 in the active sites of ?v?3 and ?(IIb)?3 integrin receptors and were compared to the results of competitive ?v?3 receptor binding assays and competitive ECV304 cell adhesion experiments. While cis RGD ligand 1 interacts mainly with the ? integrin subunit through its basic guanidine group, trans RGD ligands 2 and 3 are able to interact with both the ? and ? integrin subunits via an electrostatic clamp.
PMID: 23657523 [PubMed - indexed for MEDLINE]
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