Thread: Help!!Why does a deuterated protein behave even more poorly than the protonated one?
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Unread 11-13-2006, 04:27 PM
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The CBCA(CO)NH experiment depends on an initial 1H-13C transfer if your protein is deuterated you have no 1H's to excite on the carbons so you will get no signal, when you have a deuterated protein you have to start on the HN groups so you run a HN(CO)CACB instead.
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