Thread: NMR paper Sequential protein NMR assignments in the liquid state via sequential data acquisition.
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Default Sequential protein NMR assignments in the liquid state via sequential data acquisition.
Sequential protein NMR assignments in the liquid state via sequential data acquisition.

Related Articles Sequential protein NMR assignments in the liquid state via sequential data acquisition.

J Magn Reson. 2013 Dec 14;239C:23-28

Authors: Wiedemann C, Bellstedt P, Kirschstein A, Häfner S, Herbst C, Görlach M, Ramachandran R

Abstract
Two different NMR pulse schemes involving sequential (1)H data acquisition are presented for achieving protein backbone sequential resonance assignments: (i) acquisition of 3D {HCCNH and HNCACONH} and (ii) collection of 3D {HNCOCANH and HNCACONH} chemical shift correlation spectra using uniformly (13)C,(15)N labelled proteins. The sequential acquisition of these spectra reduces the overall experimental time by a factor of ?2 as compared to individual acquisitions. The suitability of this approach is experimentally demonstrated for the C-terminal winged helix (WH) domain of the minichromosome maintenance (MCM) complex of Sulfolobus solfataricus.


PMID: 24382576 [PubMed - as supplied by publisher]



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