Quote:
Originally Posted by bimo
Dear all,
Is that possible to do structure determination of a 30-residue peptide
using 500 MHz NMR?
I have a 30-residue linear peptide with three Gly and two AHx
(aminohexanoic acid) groups at the center of the peptide. I read some
papers from people who use 500 MHz NMR to determine the structure of
up to 20-residue peptides, but not a 30-residue peptide.
Many thanks.
Bimo
--
Bimo A Tejo, Ph.D
Department of Chemistry
Faculty of Science
Universiti Putra Malaysia
43400 UPM Serdang, Malaysia.
Tel: +60-3-8946-7488 (office) | +60-3-8946-6790 (lab)
Fax: +60-3-8943-5380
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Hi,
I did solved structure of 26 residue helical peptide and data was acquired on 700 MHz and also 500 MHz spectrometers (read this paper: Theoretical and in vitro studies of a C-terminal peptide from PGKC of Leishmania
mexicana mexicana). It depends on the spectral quality and spectral quality is depends on concentration of the sample and structure of the peptide. Higher field meagnets are better resolution and otherwise 500 MHz spectrometer is more than sufficient for 30 mer peptide.
Good Luck