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Unread 11-15-2011, 11:46 AM
krishnarjuna krishnarjuna is offline
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Default cbcanh

Hi,

Changs the buffer to 50 mM sodium phosphate or sodium acatate and salt concentration to 50 mM NaCl or Kcl. You can try with 1 mM concentrated sample. And do HNCACB insted of CBCANH. It will work. Try with low concentrated (below 1 mM) sample to overcome the problem of dimerization.

cheers, krishna
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