Thread: CBCANH experment on 700 vnmr
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Unread 05-13-2011, 09:50 PM
penumutchu.srinivas penumutchu.srinivas is offline
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Question CBCANH experment on 700 vnmr
Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion )

Iam always geting bad quality of CBCANH contures experment on 700 vnmr cryob .

quality of N-H hsqc is good

Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment
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