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[ASAP] Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar [ASAP] Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy

Keitaro Kitada, Oliver Pecher, Pieter C. M. M. Magusin, Matthias F. Groh, Robert S. Weatherup, Clare P. Grey



Journal of the American Chemical Society
DOI: 10.1021/jacs.9b01589




Source: Journal of the American Chemical Society
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Membrane Protein Characterization Service Market Growth Is Driven by High Use of Detergents Even with Best Stabilizing Power for Membrane Protein Characterization - Press Release - Digital Journal
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Membrane Protein Characterization Service Market Growth Is Driven by High Use of Detergents Even with Best Stabilizing Power for Membrane Protein Characterization - Press Release - Digital Journal

Membrane Protein Characterization Service Market Growth Is Driven by High Use of Detergents Even with Best Stabilizing Power for Membrane Protein Characterization - Press Release Digital JournalThis press release was orginally distributed by SBWire. Rockville, MD -- (SBWIRE) -- 04/23/2019 -- Membrane Protein Characterization *Service* Market Insights.

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Protein Crystallography Market 2018 Global Industry Size, Share, Business Growth, Revenue, Trends, Global Market Demand Penetration and Forecast to 2023 - Justice News Herald
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallography Market 2018 Global Industry Size, Share, Business Growth, Revenue, Trends, Global Market Demand Penetration and Forecast to 2023 - Justice News Herald

Protein Crystallography Market 2018 Global Industry Size, Share, Business Growth, Revenue, Trends, Global Market Demand Penetration and Forecast to 2023 Justice News HeraldProtein Crystallography Market Latest Research Report 2018-2023 covers the entire market structure of the whole world, with a wide range of industry analysis ...

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Fatigue in multiple sclerosis: Protein biomarkers - Ezine - spectroscopyNOW.com
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Fatigue in multiple sclerosis: Protein biomarkers - Ezine - spectroscopyNOW.com

Fatigue in multiple sclerosis: Protein biomarkers - Ezine spectroscopyNOW.comMultiple sclerosis and fatigue Fatigue is one of the most debilitating symptoms of multiple sclerosis, rendering sufferers incapable of any activity in its most ...

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Photocycle-dependent conformational changes in the proteorhodopsin cross-protomer Asp-His-Trp triad revealed by DNP-enhanced MAS-NMR [Biophysics and Computational Biology]
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Photocycle-dependent conformational changes in the proteorhodopsin cross-protomer Asp-His-Trp triad revealed by DNP-enhanced MAS-NMR [Biophysics and Computational Biology]

Jakob Maciejko, Jagdeep Kaur, Johanna Becker-Baldus, Clemens Glaubitz...
Date: 2019-04-23

Proteorhodopsin (PR) is a highly abundant, pentameric, light-driven proton pump. Proton transfer is linked to a canonical photocycle typical for microbial ion pumps. Although the PR monomer is able to undergo a full photocycle, the question arises whether the pentameric complex formed in the membrane via specific cross-protomer interactions plays... Read More


PNAS:
Number: 17
Volume: 116
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[NMR paper] pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.

Related Articles pH-dependent thermodynamic intermediates of pHLIP membrane insertion determined by solid-state NMR spectroscopy.

Proc Natl Acad Sci U S A. 2018 11 27;115(48):12194-12199

Authors: Otieno SA, Hanz SZ, Chakravorty B, Zhang A, Klees LM, An M, Qiang W


Abstract
The applications of the pH low insertion peptide (pHLIP) in cancer diagnosis and cross-membrane cargo delivery have drawn increasing attention in the past decade. With its origin as the transmembrane (TM) helix C of bacteriorhodopsin, pHLIP is also an important model for understanding how pH can affect the folding and topogenesis of a TM ?-helix. Protonations of multiple D/E residues transform pHLIP from an unstructured coil at membrane surface (known as state II, at pH >= 7) to a TM ?-helix (state III, pH
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[NMR paper] Stability of Nitroxide Biradical TOTAPOL in Biological Samples
Apr 23, 2019 - 7:54 PM - by nmrlearner
nmrlearner's Avatar Stability of Nitroxide Biradical TOTAPOL in Biological Samples

Publication date: Available online 22 April 2019

Source: Journal of Magnetic Resonance

Author(s): Kelsey McCoy, Rivkah Rogawski, Olivia Stovicek, Ann McDermott

Abstract

We characterize chemical reduction of a nitroxide biradical, TOTAPOL, used in dynamic nuclear polarization (DNP) experiments, specifically probing the stability in whole-cell pellets and lysates, and present a few strategies to stabilize the biradicals for DNP studies. DNP solid-state NMR experiments use paramagnetic species such as nitroxide biradicals to dramatically increase NMR signals. Although there is considerable excitement about using nitroxide- based DNP for detecting the NMR spectra of proteins in whole cells, nitroxide radicals are reduced in minutes in bacterial cell pellets, which we confirm and quantify here. We show that addition of the covalent cysteine blocker N-ethylmaleimide to whole cells significantly slows the rate of reduction, suggesting that cysteine thiol radicals are important to in vivo radical reduction. The use of cell lysates rather than whole cells also slows TOTAPOL reduction, which suggests a possible role for the periplasm and oxidative phosphorylation metabolites in radical degradation. Reduced TOTAPOL in lysates can also be efficiently reoxidized with potassium ferricyanide. These results point to a practical and robust set of strategies for DNP of cellular preparations.



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